PDF | The present study aims to evaluate the therapeutic properties of the medicinal plants (Vitex negundo, Aegle marmelos and Boswellia serrata) for their . Abstract To evaluate the anti-inflammatory activity of Vitex negundo (VN) leaf extract and its mechanism of action in experimental animals. Carrageenin induced. Abstract. The present study was undertaken to assess the anti-inflammatory effect of ethanolic extract of Vitex negundo roots in rats.

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Department of Pharmacognosy and Phytochemistry, Principal K. Reactive oxygen species are implicated in various inflammatory disorders. Vitex negundo is mentioned in Ayurveda as useful in treating arthritic disorders. The present work was undertaken to evaluate the antioxidant potential and anti-inflammatory activity of the plant. The total methanol extract of the plant was standardized in terms of vutex polyphenols.

The extract also exhibited a strong free radical scavenging activity by 1,1-diphenylpicrylhydrazyl method and caused a significant reduction in the formation of thiobarbituric acid reacting substances when evaluated for its lipid peroxidation inhibitory activity.

The results strongly suggest that actovity quenching may be one of the mechanisms responsible for its antiinflammatory activity.

Ayurveda mentions several plants acting as antiinflammatory agents in arthritic disorders. The tissue damage then provokes inflammatory response by the production of mediators and chemotactic factors 4. The reactive oxygen species are also known to activate matrix metalloproteinase e.

Hence the agents that can scavenge these reactive oxygen species can be beneficial in the treatment of such inflammatory disorders. The easy availability of this plant, coupled with its reported usefulness in arthritic disorders prompted us to further explore its antiinflammatory and antioxidant activity.

The plant material was collected from Versova, Mumbai and plant netundo was authenticated from Blatter Herbarium, St.

A herbarium specimen of this plant has been deposited at Principal K. Kundnani College of Pharmacy. Gum acacia, phosphoric acid, potassium ferricyanide, ferric znti were of LR grade and obtained from local suppliers.

Gallic acid and 1,1-diphenylpicrylhydrazyl was procured from Sigma Aldrich Co.

Ferrous sulphate, ascorbic acid, sodium chloride, potassium chloride, all of LR grade inflammatorry obtained from local suppliers. Diclofenac sodium was obtained from Mac Labs, Mumbai as a gift sample. The extract was concentrated under vacuum. The total polyphenolic content of the whole percolate extract was determined using Prussian activtiy method 6.


The content were mixed well and kept at room temperature for 15 min. The total polyphenols were estimated as gallic acid equivalents. This method is based on reduction in absorbance of a methanol solution of coloured free radical, 1,1-diphynylpicrylhydrazyl 7. Ascorbic acid was used as positive control. During the aerobic incubation of tissue homogenates, malondialdehydes are formed which on reaction with thiobarbituric acid produced a pink colour. The pink colour complex of viyex acid reacting substances was monitored at nm 8 — Curcumin was used as positive control.

Mice were fasted overnight before the day of experiment and the liver was promptly excised after sacrificing the mice. The liver was weighed and perfused with 0. After washing, tissue homogenate was prepared in 10 volumes of ice-cold potassium chloride by homogenizing for 15 min.

The reaction mixture contained 0. inflammqtory

Antioxidant and Antiinflammatory Activity of Vitex negundo.

To this reaction mixture thiobarbituric acid was added and the mixture was boiled for 15 min. The tubes were then centrifuged at rpm for 10 min and cooled. Absorbance of the resulting pink chromogen was recorded at nm. The antiinflammatory activity was carried out on Wistar rats using the carrageenan-induced rat paw method 11 Necessary permission was obtained from the Institutional Animal Ethics Committee.

Suspension of diclofenac sodium 6. Edema in one of the paws of the rat was induced by injecting into plantar aponeurosis 0. All vehicles, standard, and the test compound were fed to the rats one hour before the injection.

Antioxidant and Antiinflammatory Activity of Vitex negundo.

Volume of the injected paw was measured plethysmographically immediately after the injection and then again at hourly intervals up to 4h. Statistical analysis was carried out by applying student’s t-test. The whole percolate extract of V. Hence the extract was standardized in terms of total polyphenol content and anit to contain The phenolics, particularly polyphenols exhibit a wide variety of beneficial activities in mammals including antiviral, antibacterial, immune stimulating, antiallergic, antihypertensive, antiischemic, antiarrythmic, antithrombotic, hypocholesteromic, hepatoprotective, antiinflammatory, anticarcinogenic Flavonoids are an important group of polyphenols and are reported to inhibit prostaglandin synthesis, which are known mediators of inflammation When it was evaluated for its antioxidant activity by studying its lipid peroxidation inhibitory activity; it exhibited a concentration dependent inhibition of formation of thiobarbituric acid qctivity substances Table 1 with a potent antioxidant activity.

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Lipid peroxidation is responsible for damaging cell membranes thereby further intensifying inflammatory damage. A positive lipid peroxidation inhibitory activity of the extract of V. The ethno medical use of V. The present investigations have demonstrated a strong correlation between anti-inflammatory and antioxidant activities of V. The prevention of oxidative damage to tissue could therefore be one of the mechanisms responsible for the antiinflammatory effect shown by the plant.

Confirmation of the antiinflammatory activity in animal models further justifies the traditional use of this plant for inflammatory disorders. Antioxidant and antiinflammatory activity of Vitex negundo. National Center for Biotechnology InformationU.

Indian J Pharm Sci. Author information Article notes Copyright and License information Disclaimer. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

This article has been cited by other articles in PMC. Abstract Reactive oxygen species are implicated in various inflammatory disorders. Antiinflammatory activity, antioxidant activity, total polyphenol content, Vitex negundo.

Open in a separate window. Footnotes Kulkarni, et al.: Sivarajan VV, Balachandran I. Ayurvedic drugs and their plant sources. Oxidative damage and pathogenesis. Wills’ Biochemical Basis of Medicine. Anti-inflammatory Drugs from Plants and Marine sources. Robbins, Pathologic Basis of Disease. Stabilization of the Prussian blue colour in the determination of polyphenols.

J Agric Food Chem.

Antioxidant and Antiinflammatory Activity of Vitex negundo

Free Radical scavenging activity of Syzygium cumuni and Ficus bengalensis plants used in Ayurveda for Diaibetus mellitus. Effects of Luffa echinata on lipid peroxidation and free Radical scavenging Activity. Okuma, Steiner M, Baldini M. Studies on lipid peroxides in platelets: I method of assay and effects of storage. J Lab Clin Med. Assay for lipid peroxidation in animal tissues antii thiobarbituric acid reaction. Wiley Series Chemistry and Pharmacology of Drugs.

Interscience Publication, John Wiley and Sons; Non-steroidal anti-inflammatory drugs; p. Their bioactivity screening and evaluation.

Proceedings of the international workshop, Lucknow: Natural phenolic in the prevention of UV-induced skin damage: Studies on analgesic and anti inflammatory activity of Vitex negundo Linn. Support Center Support Center. Please review our privacy policy.